The Regina & District Labour Council represents more than 31,000 members from 19 unions.
- Amalgamated Transit Union, Local 588
- BCTGM, Local 389S
- Canadian Office and Professional Employees Union, Local 225
- Canadian Office and Professional Employees Union, Local 342
- Canadian Union of Public Employees, Local 7
- Canadian Union of Public Employees, Local 21
- Canadian Union of Public Employees, Local 650
- Canadian Union of Public Employees, Local 1125
- Canadian Union of Public Employees, Local 1594
- Canadian Union of Public Employees, Local 1831
- Canadian Union of Public Employees, Local 3761
- Canadian Union of Public Employees, Local 3766
- Canadian Union of Public Employees, Local 3967
- Canadian Union of Public Employees, Local 4828
- Canadian Union of Public Employees, Local 5791
- Canadian Union of Postal Workers, Local 820
- Canada Employment and Immigration Union, Local 40811
- Grain & General Services Union
- International Alliance of Theatrical Stage Employees, Moving Picture Technicians, Artists and Allied Crafts, Local 295
- International Association of Fire Fighters, Local 181
- International Brotherhood of Electrical Workers, Local 2067
- International Brotherhood of Electrical Workers, Local 2038
- Public Service Alliance of Canada, Local 40005
- Retail, Wholesale and Department Store Union, Local 454
- Retail, Wholesale and Department Store Union, Local 539
- Retail, Wholesale and Department Store Union, Local 540
- Retail, Wholesale and Department Store Union, Local 568
- Service Employees International Union West, Local 299
- Saskatchewan General and Government Employees’ Union
- Saskatchewan Union of Nurses, SDC
- UniteHere, Local 41
- United Food and Commercial Workers, Local 248 P5
- United Food and Commercial Workers, Local 1400
- United Steelworkers, Local LU5890
- United Steelworkers, Local 5917
- University of Regina Faculty Association